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A simplified system to get any Zebra fish tissue DNA ready in 30 minutes! This kit make same day genotyping the reality.
No organic solvent involved and no extra proteinase needed.
Contains 50ml Lysis Solution and 5ml DNA Stabilizing Buffer.
Zebrafish Quick Genotyping DNA Preparation Protocol
(Please wear protective gloves)
1. Place zebrafish tissue into PCR tube, 96-well PCR plate or microtubes. The paraformaldehyde-fixed embryos can also be used.
2. Add 1 volume Lysis Solution (see Table below) to each sample. Heat to 95°C for 10-20 min, then cool to 4°C. Proceed to next step immediately. Use thermo-cycler or heat-block. Typically, 10 min will be sufficient for embryos and 20 min for paraformaldehy-fixed embryos and adult tissues. The undissolved tissue does not interfere with PCR.
3. Add 1/10 volume DNA Stabilizing Buffer to each sample and mix. The sample is centrifuged to pellet the debris. 1-5μl of the supernatant is directly used for 25μl PCR reaction.
| Zebrafish tissue | Lysis solution | DNA stabilizing solution |
| Sperm | 50μl | 5μl |
| Tail | 100μl | 10μl |
| Single embryo/larvae | 20-100μl | 2-10μl |
| Eggs, multiple embryos/larvae | 100-500μl | 10-50μl |
Note:
# DNA samples should be stored at 4°C (3 month) or -20°C (>3 months)
# The DNA prepared here could not be used for Southern blot analysis.
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