Description:
GenMute™ siRNA Transfection Reagent for Primary Neurons is pre-optimized for transfecting siRNA to primary neurons. Primary neurons are notoriously heard to transfect. With the pre-optimized siRNA transfection conditions, GenMute™ reagent was confirmed to give up to 90% silencing at final 50 nM siRNA on primary cultured neurons. 

Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 25 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution

Refer to the following optimal transfection conditions for maximal silencing on primary neurons. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol