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A cartoon showing shielded GenJet™ Plus transfection reagent for in vivo DNA transfection
To eliminate the unspecific interaction between cationic nonviral vector with blood components in circulation, we utilized GenJet™ Plus reagent as backbone and cross-linked a shielding peptide (SP) with amine group of neutral lipid of the backbone. The initial testing results showed that administration of shielded GenJet™ Plus did not induce erythrocyte aggregation. Instead systematic injection of shielded GenJet™ Plus led to enhanced systematic circulation with significant improvement of renilla luciferase expression in lung, spleen and liver compared with control GenJet™ Plus without shield peptide.
Description
PepJet™ DNA In Vivo Tranfection Reagent is a novel cationic peptide based DNA delivery tool. This 28 amino-acids long peptide is accidentlly identified as a strong vector in delivering DNA in vivo. With help of protein alignment and simulation software, the peptide was synthesized, refined, screened and validated by simulating a virus protein. A shield peptide was also linked to the backbone to prevent In Vivo environment interferences, leading to high In Vivo transfection efficiency. PepJet™ was confirmed to give best renilla luciferase expression in lung, liver, spleen and kidney.
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Comparison of the delivery efficiencies to different organs in mice of a plasmid containing the Renilla luciferase gene mediated by PepJet™ reagent.
Data Sheet 
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