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HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. HapiCell™ Transfection Toxicity Removal Cocktail was developed to contain a mixture of several chemicals which were confirmed to effectively remove DNA/siRNA transfection mediated cytotoxicity. Incubation of sensitive cells (e.g., SaoS-2, HUVEC, primary kerotinocytes, etc) with HapiCell Toxicity Removal Reagent for 2 minutes at RT was confirm to completely remove the transfection related toxicity which otherwise kill the cells. The cocktail was used for chemical transfection only, not for electroporation. HapiCell™ Transfection Toxicity Removal Cocktail, 2.5 ml (x50 stock solution), is sufficient to treat 125 wells for 24-well plate and 63 wells for 6-well plate respectively.
Feature:
- Removes transfection mediated toxicity after 2 minutes incubation at RT
- Nontoxic to cells
- Maintain cell viability
- Improve transfection efficiency
- Easy to use
HapiCell™ Transfection Toxicity Removal Cocktail Effectively Remove Transfection Mediated Toxocity:
A example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on mouse skin fibroblast. A DNA encoding GFP was delivered to mouse skin fibroblasts by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (left panel) or without (right panel) HapiCell™ Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection
A example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on primary mouse kerotinocytes. A DNA encoding GFP was delivered to primary mouse kerotinocytes by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (left panel) or without (right panel) HapiCell™ Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection
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