Description

GenJet™ DNA In Vitro Tranfection Reagent for WEHI-231 is pre-optimized for transfecting WEHI-231 cells. WEHI-231 is a murine B cell lymphoma that has been used extensively as a model for the immature stage B cell and its functional response to Ag receptor cross-linking as a model for immature B cell tolerance. This cell line expresses sIgM, CD5, and FcR gamma, but lacks the B cell- specific isoform of CD45 (B220).

Refer to the following optimal transfection conditions for maximal transfection efficiency on WEHI-231 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet