The PCR Dye Master Mix II (5X) is a ready-to-use mixture for virtually all PCR applications. The mixes contain all components for PCR with the exception of template and primers. A red dye containing in the mix allow the PCR product to be direct loaded on the gel. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Dye Master Mix II (5X) is supplied as a 5X concentrated ready-to-use mix containing 0.75 u of Taq DNA polymerase, reaction buffer, 2 mM MgCl₂, 250 μM dNTP and enzyme stabilizer in a 25 μl 1X PCR mixture.Store at -20°C.