The PCR Master Mix II (5X) is a ready-to-use mixture for all PCR applications. The mixes contain all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Mix II is supplied as a 5X concentrated ready-to-use mix containing 0.75 u Taq DNA polymerase, reaction buffer, 2 mM MgCl2 , 250 μM dNTP and enzyme stabilizer in a 25 μl 1X PCR mixture. Store at -20°C