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- Ready-to-use: supplied in a loading buffer for direct loading on gels
- Easy to identify: includes the -10, -40 and -90 kDa reference bands coupled with an blue dye
- Sharp bands
Description
The PiNK Plus prestained protein ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The PiNK Plus prestained protein ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
Applications
- Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis
- Monitoring of protein transfer onto membranes during western blotting
- Sizing of proteins on SDS-polyacrylamide gels and western blots
Quality control
Tested in SDS-polyacrylamide gel electrophoresis and western blotting
Storage
Stable for up to 2 weeks at 25°C
Stable for up to 3 months at 4°C
For long term storage, store at -20°C
Recommendations for loading
1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil
2. Mix thoroughly to ensure the solution is homogeneous
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:
- 5μl per well for mini-gels, 2.5μl per well for blots
- 10 μl per well for large gels, 5μl per well for blots
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