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Key Features
• Enable streamlined protocol in a simple reaction set-up
• Allow accurate quantification of a variety of gene targets
• Reduce pipetting steps to minimize the risk of contamination
• Compatible with most real-time PCR instruments
Please refer to our qPCR MasterMix Selection Guide (found in theDocuments section below) for selecting the appropriate qPCR formulation applicable to your particular instrument model.Literature
Application•
Gene-expression analysis
• Gene knockdown validation
• SNP genotyping assays
• CHiP
• Copy number variation
• Microarray validation
• High throughput applications
• Virus detection and quantificationPicture 1
ProtocolStorageStored at -20°C and protect from light.Notes1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
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