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| Description | Convenient real-time RNA quantification in one-step. abm’s One-Step EvaGreen qRT-PCR Kit uses a combination of high-quality enzymes in a proprietary buffer system to deliver precise and accurate sample analysis in a high-throughput format. This kit offers ultimate convenience to the end-user in addition to guaranteed performance with respect to high sensitivity, low signal-to noise ratio, and complete elimination of primer dimmers. Key Features • Streamlined protocol in a simple single-tube reaction set-up • High-quality, full-length cDNA from as little as 0.01pg of RNA • Fully optimized for detection of low-copy genes • Simple set-up for any RNA template • Reduces pipetting steps to minimize the risk of contamination Please refer to our EvaGreen qPCR MasterMix Selection Guide in selecting the appropriate qPCR formulation applicable to your particular instrument model. |
| Application | • Gene-expression analysis • Transcription analysis • Gene cloning • High throughput applications • Virus detection and quantification |
| Form | This kit includes 50X qRT-PCR Enzyme Mix and EvaGreen qPCR MasterMix |
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| Storage | Stored all components at -20°C. Protect the EvaGreen qPCR MasterMix from light. |
| Notes | 1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles. 2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results. 3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range. This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
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