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| Description | Synthesize cDNA from only 0.1pg of RNA with abm’s EasyScript™ Reverse Transcriptase. Using strategic modifications, abm’s EasyScript™ Reverse Transcriptase lacks the intrinsic RNase H acitivity thereby drastically increases the yield and achievable synthesized cDNA length (up to 9kb). In addition, EasyScript™ contains a fidelity-enhancing subunit which ensures superior accuracy in reverse transcription reaction(s). |
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| Application | • Synthesizing cDNA from a ssRNA • DNA primer extension • Sequencing dsDNA • Constructing cDNA library • Constructing libraries for serial analysis for gene expression (SAGE) • Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE) • Producing template for use in RT-PCR or real-time RT-PCR • Labelling 3'-end of duplex DNA via end-filling reactions • Generating probes for hybridization. |
| Form | Enzyme supplied with 5X RT buffer |
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| Protocol | |
| Concentration | 200 U/ul |
| Storage | Store all components at -20°C. |
| Notes | This product is distributed for laboratory research only. Caution: Not for diagnostic use . |
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