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| Description | Protease Inhibitor Cocktail is used to prevent the digestion of proteins that occurs after cell lysis takes place. |
| Composition | 100mM PMSF, 2mM Bestatin, 0.3mM Pepstatin A, and 0.3mM E-64 |
| Protocol | 1mL of the Protesase Inhibitor Cocktail is recommended for the inhibition of protease activity form 100mL of cell lysate at a density of 10^8 cells per mL.
However, since not all organisms contain the same level of endogenous proteases, it is up to the researcher to optimize the concentration for their experiments. |
| Storage | Store cocktail at -20°C. Cocktail is dissolved in water. The cocktail may form crystals. If there are crystals, place it at RT for 5 minutes. The cocktail is stable for a year. |
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