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| Description | Deoxyribonuclease I (DNase I; EC3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.1,2 The catalytic activity of DNase I entails an obligate requirement for Ca2+ as its metal cofactor while Mg2+ ions offer a synergistic component to the reaction milieu.3 DNase I is synonymous with Pancreatic DNase, Endodeoxyribonuclease I and Thymonuclease. |
| Manual |  |
| Application | - Degrade template DNA following in vitro transcription - Remove contaminating genomic DNA from protein samples - Mediate nick translation - Reduce viscosity of cell lysates and prevent clumping when handling cultured cells - Mediate DNase I foot-printing |
| Storage | 2mM CaCl2,10mM Tris-HCl (pH 7.6) and 50% glycerol. Store at -20°C. |
| Handling | Shipped with dry ice |
| Reference | (1) Kunitz, M (1950). J. Gen. Physiol. 33, 349-362. (2) Vanecko, S and Laskowski, M (1961). J Biol Chem 236: 3312-3316. (3) Moores, S (1981). Pancreatic DNase, in: The Enzymes (P.D. Boyer, Ed.) Academic Press, New York, Chapter 15. |
| Notes | One unit is defined as the amount of enzyme which will completely degrade 1μg of pBR322 DNA in 10 mins at 37 °C. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller. |
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