Problems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase is chemically modified such that the Taq enzyme is inactivated by a heat-labile group at room temperature. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, abm’s HotStart DNA Polymerase offers improved yield of desired PCR products.
With abm’s HotStart DNA Polymerase, you can achieve
• The highest specificity with minimal background
• Superior performance
• Improved yield of desired product
Literature
Application
• Assays with prolonged reaction setup or liquid handling
• Multiplex PCR
• Specific amplification of difficult templates (i.e. G-C rich)
• Low copy PCR assays
• TA Cloning
Form
Enzyme supplied with 10X buffer and MgSO4
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Protocol
Concentration
5 U/µl
Storage
Store all components at -20°C.
Notes
This product is distributed for laboratory research only.
Caution: Not for diagnostic use .