Krueppel-like factor 4 (KLF4) is a zinc finger transcriptional regulator that has been identified as a good indicator of stem cell-like capacity and is subsequently used in iPS cell generation. KFL4 plays an important role in maintaining stem cells, and in preventing their differentiation.
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. EF-1 alpha (or EF1a) is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells).
Virus: |
EF1A-KLF4 |
Titer (approx): |
1 x 108 CFU/ml |
Vector Information: |
Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) |
Promoter for Target Gene: |
EF1A |
Target Gene / Reporter(s): |
KLF4 |
Selection Gene: |
Puromycin |
Biosafety Level: |
BSL-2 |
Shipped: |
Dry ice |
Storage: |
Store at -80°C |