Description

The DM3100 ExcelBand 1 KB (0.25-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA ladder DM3100 is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easy referencing. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.

Source

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range

250 ~ 10,000 bp

Concentration

50 µg/ 500 µl

Recommended loading volume

5 µl/ well

Storage

Room temperature  ≥ 6 months

                       4°C ≥ 12 months

                    -20°C ≥ 36 months

DM3100 MSDS DM3100 Product Information