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OneMARK B with the Novel Green was designed to show virtually uniform spacing over a wide fragment
range. The ladder is supplied in a ready-to-use format containing the fluorescent DNA stain and tracking
dyes. High quantum yield and excellent stability make the fluorescence dye the ideal fluorophore for
DNA staining applications and a superior replacement for the widely used dyes, ethidium bromide or
SYBR® Green I.
The OneMARK B with the Novel Green was optimized for direct loading onto unstained agarose gels.
The ladders provide the highest level of convenience during the routine handling and avoid commonly
used gel staining procedures with the ethidium bromide or SYBR® Green I.
The OneMARK B includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have
increased intensity to serve as reference points. The approximate mass of DNA in each band is provided
(0.5 μg per loading) for approximating the mass of DNA in comparably intense samples of similar size.
Application
- No-post-staining procession
- Direct loading onto your agarose gel for analysis
Source
PCR products and double-stranded DNA digested with
appropriate restriction enzymes are phenol-extracted and
equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Note
OneMARK B is light sensitive and should be stored and
protected from light.
Range: 250-10,000 bp
Number of bands: 13
Concentration: 83.3 μg/ml
Package: 50 μg /600 μl
Recommended Load: 6 μl / well
Containing bromophenol blue and xylene cyanol FF
as the tracking dyes.
Storage
Store at RT and 4°C up to 6 month.
Store at -20°C up to 1 year.
Size: 600 μl
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