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OneMARK 100 with the Novel Green was designed to show virtually uniform spacing over a wide
fragment range. The ladder is supplied in a ready-to-use format containing the fluorescent DNA stain
and tracking dyes. High quantum yield and excellent stability make the fluorescence dye the ideal
fluorophore for DNA staining applications and a superior replacement for the widely used dyes, ethidium
bromide or SYBR® Green I.
The OneMARK 100 with the Novel Green was optimized for direct loading onto unstained agarose gels.
The ladders provide highest level of convenience during the routine handling and avoid commonly used
gel staining procedures with ethidium bromide or SYBR® Green I.
The OneMARK 100 includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair
bands have increased intensity to serve as reference points. The approximate mass of DNA in each band
is provided (0.54 μg per loading) for approximating the mass of DNA in comparably intense samples of
similar size.
Application
- No-post-staining procession
- Direct loading onto your agarose gel for analysis
Source
PCR products and double-stranded DNA digested with
appropriate restriction enzymes are phenol-extracted and
equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Note:
OneMARK 100 is light sensitive and should be stored and
protected from light.
Range: 100-3,000 bp
Number of bands: 12
Concentration: 90 μg/ml
Package: 54 μg /600 μl
Recommended Load: 6 μl / well
Containing orange G, xylene cyanol FF as the tracking dyes.
Storage
Store at RT and 4°C up to 6 month.
Store at -20°C up to 1 year.
Size:600 μl
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