Induced pluripotent stem (iPS) cells can be generated from differentiated somatic cells by over-expressing a set of specific transcription factors called reprogramming factors. Typically four reprogramming factors are chosen, Oct4, Sox2, Klf4 and c-Myc (OSKM), which are introduced into the target cells through genetic transduction using retroviral or lentiviral vectors. This product packages all four genes (OSKM) in one lentiviral vector.
Virus: |
CMV-OSKM |
Titer (approx): |
1 x 108 TU/ml |
Vector Information: |
Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) |
Promoter for Target Gene: |
CMV |
Target Gene / Reporter(s): |
OCT4, SOX2, KLF4, C-MYC |
Selection Gene: |
Puromycin |
Biosafety Level: |
BSL-2 |
Shipped: |
Dry ice |
Storage: |
Store at -80°C |