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This is a thermostable DNA polymerase isolated from a strain of Thermus sp. designed for primer-extension reactions. Tsg is more stable than many other DNA polymerases, with a half-life of 3 hours at 95°C. Like Taq DNA Polymerase, Tsg has an independent terminal transferase activity, which results in the addition of a single adenosine at the 3′ end of the PCR products, so that TA cloning vectors can be used.
SPECIAL FEATURES
•Excellent yields
•Alternative for Taq Polymerase
•Greater stability
•Best value
APPLICATIONS
•General PCR reactions
•High-throughput PCR
•Molecular cloning
Comparison of an amplified 1.3kb mouse gene fragment using Tsg and Taq DNA Polymerases.
•Lane M: 1Kb DNA ladder
•Lanes 1 and 2: Taq DNA polymerase from Company R
•Lanes 3 and 4: Taq DNA polymerase from Company Q
•Lanes 5 and 6: Lamda Biotech's Tsg DNA Polymerase
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